Polymorphism Exploration of Growth Family (GH, GHRH and PIT-1) Genes Polymorphisms of Local Swamp Buffalo for Productivity Improvement in North Tapanuli Regency, North Sumatra

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. polymorphism family (GH, GHRH, and PIT1) were studied in local swamp buffalo (106 hds.) from a government breeding station (46 heads) smallholders (60 North Tapanuli District, Sumatra Province. Genotype variants three identified by PCR-RFLP method using restriction enzymes MspI (GH gene), HaeIII (GHRH gene) HinfI (PIT -1 gene). Genotyping individual GH_g.1547T>C, GHRH_g.4666G>C, PIT -1_g.1256G >A loci resulted only one type genotype, respectively TT, CC, an d AA, with allele, T, C, A. Heterozygosity observation (Ho) expectation (He) values PIC value for each locus was 0.00. It could suggested to increase genotype frequenciest that are positively associated traits economic buffalo.